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Location: Bekasi, Jawa Barat, Indonesia

Wednesday, May 5, 2010

Anthurium Vegetative propagation

Division and cutting are the commonly used methods involved in vegetative propagation.

1. Division
Anthurium can easily be propagated by division of off- shoots with aerial roots from the main stem. Plants obtained through this method flower early in comparison with those from cuttings or other methods. Cultivars belonging to A. scherzerianum generally produce more number of offshoots as compared to those of A. andreanum.

2. Cutting
Anthuriums are also propagated by terminal cuttings rooted under intermittent mist. Mist accelerates rooting and enhances the survival. Plant age has been found to have no marked effect on rooting but more leaves are produced by rooted cuttings taken from the older plants. Seradix 1, a hormone preparation give the best rooting while reduction of the transpiration area has no effect on root formation or top growth.
C. Micro-propagation
With tissue culture techniques, anthuriums are multiplied very rapidly and flower production has been reported to be higher than those from seedlings. A modified Murashige and Skoog's medium (MS) has been suggested by several workers. The growth of callus tissue from adult A. andreanum plants is found to be the best in such medium. Both A. andreanum and A. scherzerianum explants produces many shoots when cultivated 'in vitro' in the presence of Benzyladenine (BA) and 2,4- D. However, yeast extract stimulates shoot growth of A. scherzerianum but restricts it in A. andreanum. Pot plants A. scherzerianum could be obtained more quickly than those of A. andreanum owing to the former's rapid callus formation and shoot growth.

Regeneration of leaf explants been found be better than other plant parts. A method of propagation that can be used in commercial practice has been suggested. After callus has formed on pieces of leaf tissue it is transferred to a solid medium. When the callus starts growth it is transferred to a liquid medium in Erlenmeyer flask which is placed on a shaking machine rotating at 120 revolutions a minute.
All the tested cultivars in this medium grew well but there will be marked differences in growth and vigour among them is noticed. To obtain plantlets the callus clumps are transferred again to a solid medium in the dark, where shoots are formed, these shoots are later rooted in light. Multiplication by this method is far more rapid than with the use of only solid agar medium. Disinfection of the tissues for 20 minutes results in less damage than disinfection for 30 minutes.

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