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Location: Bekasi, Jawa Barat, Indonesia

Monday, March 23, 2009

Genetic Modification

Genetic modification of plants is achieved by adding a specific gene or genes to a plant, or by knocking out a gene with RNAi, to produce a desirable phenotype. The plants resulting from adding a gene are often referred to as transgenic plants. If for genetic modification genes of the species or of a crossable plant are used under control of their native promoter, then they are called Cisgenic plants. Genetic modification can produce a plant with the desired trait or traits faster than classical breeding because the majority of the plant's genome is not altered.
To genetically modify a plant, a genetic construct must be designed so that the gene to be added or removed will be expressed by the plant. To do this, a
promoter to drive transcription and a termination sequence to stop transcription of the new gene, and the gene or genes of interest must be introduced to the plant. A marker for the selection of transformed plants is also included. In the laboratory, antibiotic resistance is a commonly used marker: plants that have been successfully transformed will grow on media containing antibiotics; plants that have not been transformed will die. In some instances markers for selection are removed by backcrossing with the parent plant prior to commercial release.
The construct can be inserted in the plant genome by
genetic recombination using the bacteria Agrobacterium tumefaciens or A. rhizogenes, or by direct methods like the gene gun or microinjection. Using plant viruses to insert genetic constructs into plants is also a possibility, but the technique is limited by the host range of the virus. For example, Cauliflower mosaic virus (CaMV) only infects cauliflower and related species. Another limitation of viral vectors is that the virus is not usually passed on the progeny, so every plant has to be inoculated.
The majority of commercially released transgenic plants, are currently limited to plants that have introduced resistance to
insect pests and herbicides. Insect resistance is achieved through incorporation of a gene from Bacillus thuringiensis (Bt) that encodes a protein that is toxic to some insects. For example, the cotton bollworm, a common cotton pest, feeds on Bt cotton it will ingest the toxin and die. Herbicides usually work by binding to certain plant enzymes and inhibiting their action. The enzymes that the herbicide inhibits are known as the herbicides target site. Herbicide resistance can be engineered into crops by expressing a version of target site protein that is not inhibited by the herbicide. This is the method used to produce glyphosate resistant crop plants (See Glyphosate)
Genetic modification of plants that can produce
pharmaceuticals (and industrial chemicals), sometimes called pharmacrops, is a rather radical new area of plant breeding.

Classical Plant Breeding

Classical plant breeding uses deliberate interbreeding (crossing) of closely or distantly related individuals to produce new crop varieties or lines with desirable properties. Plants are crossbred to introduce traits/genes from one variety or line into a new genetic background. For example, a mildew-resistant pea may be crossed with a high-yielding but susceptible pea, the goal of the cross being to introduce mildew resistance without losing the high-yield characteristics. Progeny from the cross would then be crossed with the high-yielding parent to ensure that the progeny were most like the high-yielding parent, (backcrossing). The progeny from that cross would then be tested for yield and mildew resistance and high-yielding resistant plants would be further developed. Plants may also be crossed with themselves to produce inbred varieties for breeding.
Classical breeding relies largely on
homologous recombination between chromosomes to generate genetic diversity. The classical plant breeder may also makes use of a number of in vitro techniques such as protoplast fusion, embryo rescue or mutagenesis (see below) to generate diversity and produce hybrid plants that would not exist in nature.

Traits that breeders have tried to incorporate into crop plants in the last 100 years include:
1. Increased
quality and yield of the crop
2. Increased
tolerance of environmental pressures (salinity, extreme temperature, drought)
3. Resistance to
viruses, fungi and bacteria
4. Increased tolerance to
insect pests
5. Increased tolerance of
herbicides

Domestication

Plant breeding in certain situations may lead the domestication of wild plants. Domestication of plants is an artificial selection process conducted by humans to produce plants that have more desirable traits than wild plants, and which renders them dependent on artificial (usually enhanced) environments for their continued existence. The practice is estimated to date back 9,000-11,000 years. Many crops in present day cultivation are the result of domestication in ancient times, about 5,000 years ago in the Old World and 3,000 years ago in the New World. In the Neolithic period, domestication took a minimum of 1,000 years and a maximum of 7,000 years. Today, all of our principal food crops come from domesticated varieties. Almost all the domesticated plants used today for food and agriculture were domesticated in the centers of origin. In these centers there is still a great diversity of closely related wild plants, the so called crop wild relatives that can also be used for improving modern cultivars by plant breeding.
A plant whose origin or selection is due primarily to intentional human activity is called a
cultigen, and a cultivated crop species that has evolved from wild populations due to selective pressures from traditional farmers is called a landrace. Landraces, which can be the result of natural forces or domestication, are plants (or animals) that are ideally suited to a particular region or environment. An example are the landraces of rice, Oryza sativa subspecies indica, which was developed in South Asia, and Oryza sativa subspecies japonica, which was developed in China.

Plant Breeding

Plant breeding is the art and science of changing the genetics of plants for the benefit of humankind. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques.
Plant breeding has been practiced for thousands of years, since near the beginning of human civilization. It is now practiced worldwide by individuals such as gardeners and farmers, or by professional plant breeders employed by organizations such as government institutions, universities, crop-specific industry associations or research centers. ..
International development agencies believe that breeding new crops is important for ensuring food security by developing new varieties that are higher-yielding, resistant to pests and diseases, drought-resistant or regionally adapted to different environments and growing conditions

Thursday, March 5, 2009

Health effects

Pesticides can present danger to consumers, bystanders, or workers during manufacture, transport, or during and after use.
The American Medical Association recommends limiting exposure to pesticides and using safer alternatives:
Particular uncertainty exists regarding the long-term effects of low-dose pesticide exposures. Current surveillance systems are inadequate to characterize potential exposure problems related either to pesticide usage or pesticide-related illnesses…Considering these data gaps, it is prudent…to limit pesticide exposures…and to use the least toxic chemical pesticide or non-chemical alternative

Environmental effects

Pesticide use raises a number of environmental concerns. Over 98% of sprayed insecticides and 95% of herbicides reach a destination other than their target species, including non-target species, air, water, bottom sediments, and food.Pesticide drift occurs when pesticides suspended in the air as particles are carried by wind to other areas, potentially contaminating them. Pesticides are one of the causes of water pollution, and some pesticides are persistent organic pollutants and contribute to soil contamination

Regulation

In most countries, in order to sell or use a pesticide, it must be approved by a government agency.For example, in the United States, the Environmental Protection Agency (EPA) does so. Complex and costly studies must be conducted to indicate whether the material is safe to use and effective against the intended pest. During the registration process, a label is created which contains directions for the proper use of the material. Based on acute toxicity, pesticides are assigned to a Toxicity Class.
Some pesticides are considered too
hazardous for sale to the general public and are designated restricted use pesticides. Only certified applicators, who have passed an exam, may purchase or supervise the application of restricted use pesticides. Records of sales and use are required to be maintained and may be audited by government agencies charged with the enforcement of pesticide regulations.
In Europe, recent EU legislation has been approved banning the use of highly toxic pesticides including those which are
carcinogenic, mutagenic or toxic to reproduction, those which are endocrine-disrupting, and those which are persistent, bioaccumulative and toxic (PBT) or very persistent and very bioaccumulative (vPvB). Measures were approved to improve the general safety of pesticides across all EU member states.

History

Since before 2500 BCE, humans have utilized pesticides to protect their crops. The first known pesticide was elemental sulfur dusting used in Sumeria about 4,500 years ago. By the 15th century, toxic chemicals such as arsenic, mercury and lead were being applied to crops to kill pests. In the 17th century, nicotine sulfate was extracted from tobacco leaves for use as an insecticide. The 19th century saw the introduction of two more natural pesticides, pyrethrum which is derived from chrysanthemums, and rotenone which is derived from the roots of tropical vegetables.
In 1939, Paul Müller discovered that DDT was a very effective insecticide. It quickly became the most widely-used pesticide in the world.
In the 1940s manufacturers began to produce large amounts of synthetic pesticides and their use became widespread.
Some sources consider the 1940s and 1950s to have been the start of the "pesticide era. Pesticide use has increased 50-fold since 1950 and 2.3 million tonnes (2.5 million short tons) of industrial pesticides are now used each year.Seventy-five percent of all pesticides in the world are used in developed countries, but use in developing countries is increasing.
In the 1960s, it was discovered that DDT was preventing many fish-eating birds from reproducing, which was a serious threat to biodiversity. Rachel Carson wrote the best-selling book Silent Spring about biological magnification. The agricultural use of DDT is now banned under the Stockholm Convention on Persistent Organic Pollutants, but it is still used in some developing nations to prevent malaria and other tropical diseases by spraying on interior walls to kill or repel mosquitoes.

Uses

Pesticides are used to control organisms which are considered harmful.For example, they are used to kill mosquitoes that can transmit potentially deadly diseases like west nile virus, yellow fever, and malaria. They can also kill bees, wasps or ants that can cause allergic reactions. Insecticides can protect animals from illnesses that can be caused by parasites such as fleas.Pesticides can prevent sickness in humans that could be caused by mouldy food or diseased produce. Herbicides can be used to clear roadside weeds, trees and brush. They can also kill invasive weeds in parks and wilderness areas which may cause environmental damage. Herbicides are commonly applied in ponds and lakes to control algae and plants such as water grasses that can interfere with activities like swimming and fishing and cause the water to look or smell unpleasant. Uncontrolled pests such as termites and mould can damage structures such as houses.Pesticides are used in grocery stores and food storage facilities to manage rodents and insects that infest food such as grain. Each use of a pesticide carries some associated risk. Proper pesticide use decreases these associated risks to a level deemed acceptable by pesticide regulatory agencies such as the United States Environmental Protection Agency (EPA) and the Pest Management Regulatory Agency (PMRA) of Canada.
Pesticides can save farmers' money by preventing crop losses to insects and other pests; in the US, farmers get an estimated fourfold return on money they spend on pesticides.
One study found that not using pesticides reduced crop yields by about 10%. Another study,conducted in 1999, found that a ban on pesticides in the United States may result in a rise of food prices, loss of jobs, and an increase in world hunger.
DDT, sprayed on the walls of houses, is an organochloride that has been used to fight malaria since the 1950s. Recent policy statements by the World Health Organization have given stronger support to this approach. Dr. Arata Kochi, WHO's malaria chief, said, "One of the best tools we have against malaria is indoor residual house spraying. Of the dozen insecticides WHO has approved as safe for house spraying, the most effective is DDT. However, since then, an October 2007 study has linked breast cancer from exposure to DDT prior to puberty.Poisoning may also occur due to use of DDT and other chlorinated hydrocarbons by entering the human food chain when animal tissues are affected. Symptoms include nervous excitement, tremors, convulsions or death. Scientists estimate that DDT and other chemicals in the organophosphate class of pesticides have saved 7 million human lives since 1945 by preventing the transmission of diseases such as malaria, bubonic plague, sleeping sickness, and typhus.However, DDT use is not always effective, as resistance to DDT was identified in Africa as early as 1955, and by 1972 nineteen species of mosquito worldwide were resistant to DDT.A study for the World Health Organization in 2000 from Vietnam established that non-DDT malaria controls were significantly more effective than DDT use.The ecological effect of DDT on organisms is an example of bioaccumulation.

Types of pesticides

There are multiple ways of classifying pesticides.
Algicides or algaecides for the control of algae
Avicides for the control of birds
Bactericides for the control of bacteria
Fungicides for the control of fungi and oomycetes
Herbicides for the control of weeds
Insecticides for the control of insects - these can be ovicides (substances that kill eggs), larvicides (substances that kill larvae) or adulticides (substances that kill adults)
Miticides or acaricides for the control of mites
Molluscicides for the control of slugs and snails
Nematicides for the control of nematodes
Rodenticides for the control of rodents
Virucides for the control of viruses (e.g. H5N1)
Pesticides can also be classed as synthetic pesticides or
biological pesticides (biopesticides), although the distinction can sometimes blur.
Broad-spectrum pesticides are those that kill an array of species, while narrow-spectrum, or selective pesticides only kill a small group of species.

A systemic pesticide moves inside a plant following absorption by the plant. With insecticides and most fungicides, this movement is usually upward (through the xylem) and outward. Increased efficiency may be a result. Systemic insecticides which poison pollen and nectar in the flowers may kill needed pollinators such as bees.
Most pesticides work by
poisoning pests.

Pesticide

A pesticide is a substance or mixture of substances used to kill a pest.[1] A pesticide may be a chemical substance, biological agent (such as a virus or bacteria), antimicrobial, disinfectant or device used against any pest. Pests include insects, plant pathogens, weeds, molluscs, birds, mammals, fish, nematodes (roundworms) and microbes that compete with humans for food, destroy property, spread or are a vector for disease or cause a nuisance. Although there are benefits to the use of pesticides, there are also drawbacks, such as potential toxicity to humans and other animals.

Wednesday, March 4, 2009

Pesticides And Farmers

There are studies detailing the effects and side effects of pesticides upon the health of farm workers.Even when pesticides are used correctly, they still end up in the air and bodies of farm workers. Through these studies, organophosphate pesticides have become associated with acute health problems such as abdominal pain, dizziness, headaches, nausea, vomiting, as well as skin and eye problems.In addition, there have been many other studies that have found pesticide exposure is associated with more severe health problems such as respiratory problems, memory disorders, dermatologic conditions,cancer,depression, neurologic deficits,miscarriages, and birth defects.Summaries of peer-reviewed research have examined the link between pesticide exposure and neurological outcomes and cancer in organophosphate-exposed workers.
Imported fruits and vegetables from South America are more likely to contain high level of pesticides, even pesticides banned for use in the United States.Migratory birds, such as Swainson's hawks, have wintering grounds in Argentina where thousands of them were found dead from monocrotophos insecticide poisoning

Identifying OrganicFood

Organic Food: A Public Discussion Why are people consuming organic food?

Mixed organic bean sprouts
Processed organic food usually contains only organic ingredients. If non-organic ingredients are present, at least a certain percentage of the food's total plant and animal ingredients must be organic (95% in the United States
[4] and Australia) and any non-organically produced ingredients are subject to various agricultural requirements. Foods claiming to be organic must be free of artificial food additives, and are often processed with fewer artificial methods, materials and conditions, such as chemical ripening, food irradiation, and genetically modified ingredients.
They may also be required to be produced using energy-saving technologies and packaged using recyclable or biodegradable materials when possible.[
citation needed]
Early consumers interested in organic food would look for non-chemically treated, fresh or minimally processed food. They mostly had to buy directly from growers: "Know your farmer, know your food" was the motto. Personal definitions of what constituted "organic" were developed through firsthand experience: by talking to farmers, seeing farm conditions, and farming activities. Small farms grew vegetables (and raised livestock) using
organic farming practices, with or without certification, and the individual consumer monitored. As demand for organic foods continues to increase, high volume sales through mass outlets such as supermarkets are rapidly replacing the direct farmer connection. However, for supermarket consumers, food production is not easily observable, and product labeling, like "certified organic", is relied on. Government regulations and third-party inspectors are looked to for assurance. A "certified organic" label is usually the only way for consumers to know that a processed product is "organic".

Tuesday, March 3, 2009

Organic Food

It has been suggested that Beyond organic be merged into this article or section. (Discuss)

Organic vegetables at a farmers' market in Argentina.
Organic foods are made according to certain production standards, meaning they are grown without the use of conventional
pesticides and artificial fertilizers, free from contamination by human or industrial waste, and processed without irradiation or food additives.[1] If livestock are involved, they must be reared without the routine use of antibiotics and without the use of growth hormones, and generally fed a healthy diet. In most countries, organic produce may not be genetically modified.
Organic food production is a heavily regulated industry, distinct from private gardening. Currently, the European Union, the United States, Canada, Japan and many other countries require producers to obtain
special certification in order to market food as "organic" within their borders. Most certifications allow some chemicals and pesticides to be used, so consumers should be aware of the standards for qualifying as "organic" in their respective locales.
Historically,
organic farms have been relatively small family-run farms[2] — which is why organic food was once only available in small stores or farmers' markets. However, since the early 1990s organic food production has had growth rates of around 20% a year, far ahead of the rest of the food industry, in both developed and developing nations. As of April 2008, organic food accounts for 1-2% of food sales worldwide.

About My Faculty

I was studied in Gadjah Mada University and take Agriculture Faculty. If you want to choose this Faculty, please think about it again. This Faculty is not profesional, because there is uncomputerized if we take a main lesson and must to meet a lecturer to get sign and then we must go to computer to input our main lesson. Not simple and wasting time. The class can unavailable if we late to input our main lesson. If our lecturer not in her/his room, we must wait her/him. It can be we can not get our class because the class is full. To new student, please thinking again. To our Faculty, please change this system to get best.

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